为研究单一及混合碳源、氮源对兽疫链球菌生长的影响，以葡萄糖、果糖、蔗糖、木糖、半乳糖、麦芽糖和红糖作为碳源，以酵母膏、牛肉膏、蛋白胨、玉米浆干粉、大豆粉、（NH₄）₂SO₄作为氮源，进行不同条件的兽疫链球菌发酵培养，测定菌产量，观察菌的形态学特征。结果表明，单一碳源葡萄糖培养条件下，菌株生长较好，产量为4.86 g/L，混合碳源培养条件下兽疫链球菌的产量较低。酵母膏作为单一氮源时，兽疫链球菌的产量最高，为4.86 g/L；酵母膏和蛋白胨混合时，兽疫链球菌产量为5.14 g/L，酵母膏+牛肉膏+蛋白胨混合时，产量为5.33 g/L。菌株形态学特征表明，其符合典型兽疫链球菌特性。综合表明，以葡萄糖为单一碳源时兽疫链球菌生长较佳，而采用多重混合氮源培养时，兽疫链球菌的产量高于混合氮源和单一氮源体系。

To investigate the effects of single or composed carbon and nitrogen sources on the growth of Streptococcus zooepidemicus, fermentation cultures were conducted using glucose, fructose, sucrose, xylose, galactose, maltose, and brown sugar as carbon sources, and yeast paste, beef paste, peptone, corn steep liquor powder, soybean meal, and (NH₄)₂SO₄ as nitrogen sources under varying conditions. Bacterial yield was measured, and morphological characteristics were observed.The results showed that under single carbon source (glucose) conditions, the strain exhibited better growth with a yield of 4.86 g/L, whereas mixed carbon sources resulted in lower yields. When yeast paste was used as the sole nitrogen source, the highest bacterial yield reached 4.86 g/L. The combination of yeast paste and peptone yielded 5.14 g/L, while the mixture of yeast paste + beef paste + peptone further increased the yield to 5.33 g/L. The morphological characteristics of the strain indicated that it conformed to the typical characteristics of Streptococcus zooepidemicus. In summary, Streptococcus zooepidemicus demonstrated optimal growth when glucose was used as the sole carbon source, while cultivation with multiple mixed nitrogen sources led to higher yields compared to both mixed and single nitrogen source systems.