To understand the occurrence of diseases and pests in greenhouse strawberry in Lijiang City, Yunnan Province, strawberry disease and pest investigations were carried out at three experimental sites from September 2023 to August 2004 using the random five-point sampling method. The growth status and the occurrence of diseases and pests on strawberry plants were recorded. During the peak occurrence period of diseases and pests, observations were carried out every 7 days, and the incidence rate, disease index, percentage of infested plants, and pest index were documented. The results showed that the main diseases and pests of greenhouse strawberries in the study area included gray mold, powdery mildew, anthracnose, root rot, aphids and spider mites. In terms of occurrence status, the disease indices of gray mold, powdery mildew, root rot and anthracnose at experimental site 2 were 46.50, 40.66, 36.67 and 34.07, respectively; those at experimental site 3 were 40.83, 36.33, 35.80 and 34.55, respectively; and those at experimental site 1 were 33.17, 35.83, 29.15 and 29.92, respectively. The insect indices of spider mites and aphids at experimental site 2 were 38.18 and 32.67, respectively; those at experimental site 3 were 31.60 and 34.00, respectively; and those at experimental site 1 were 30.74 and 28.50, respectively. In terms of infection regularity, gray mold infested all above-ground parts of strawberry plants, with the most severe damage to fruits. Powdery mildew mostly infected strawberry leaves and fruits by mycelia or conidia, and could also damage petioles, calyxes and pedicels in severe cases. Red stele root rot mainly damaged strawberry roots, and severe infection would cause root rot and subsequent whole plant death. Adult aphids sucked sap from tender heart leaves, leaf undersides and petioles, resulting in twisting, deformation and curling of strawberry leaves. Spider mites mostly gathered on the leaf undersides of strawberry; at the early infestation stage, white spots appeared on the leaves and gradually turned red, and the leaves showed a rust-colored appearance in severe cases. Growth conditions (e.g., temperature, humidity, ventilation), strawberry cultivars (e.g., yield potential, disease and pest resistance) and cultural management (e.g., fertilizer and water management, cultivation management) all had certain effects on the occurrence severity of diseases and pests. In terms of disease and pest control, targeted measures should be adopted according to local conditions, and agricultural, physical, biological and chemical control measures should be comprehensively applied to control diseases such as gray mold and powdery mildew, as well as insect pests such as spider mites and aphids. This study provides a reference for the investigation, control and prevention of strawberry diseases and pests in similar areas.