针对当前细胞生物学实验课程在教学模式、实验内容、课前准备及考核方式等方面存在的提升空间，本文分析了该课程的教学现状，结合教学实际开展教学改革实践。改革围绕丰富教学模式、优化实验内容、加强课前准备、完善考核标准4大维度展开。具体包括升级实验室硬件设施、依托网络资源拓展学习维度；依据理论课程重难点修订教学方案，优化设置线粒体的超活染色与观察等9个实验项目，兼顾课程验证性、综合性与研究性；规范试剂配制、材料准备、仪器调试流程，引导学生参与课前准备并强化安全规范；构建过程性与结果性相结合的多元化考核体系，明确量化评分标准。教学实践表明，改革已取得阶段性成效，获批大学生创新创业训练计划项目23项，逐步完善了实验教学与考核评价体系，有效提升了学生的实验操作能力、创新实践能力及课程满意度。本文为同类农业院校细胞生物学及相关专业实验课程的教学改革提供参考。

To address the areas for improvement in the teaching mode, experimental content, pre-class preparation, and assessment methods of the current Cell Biology Experiment course, this study analyzed the present teaching situation and carried out teaching reform practices based on actual teaching conditions. The reform focused on 4 aspects: enriching teaching modes, optimizing experiment content, strengthening pre-class preparation, and improving assessment standards. Specifically, laboratory hardware facilities were upgraded and network resources were used to expand learning dimensions. The teaching plan was revised according to the key and difficult points of the theoretical course, and 9 experiment items, including mitochondrial supravital staining and observation, were optimized, covering confirmative, comprehensive and research-oriented experiments. The procedures of reagent preparation, material preparation and instrument debugging were standardized, students were guided to participate in pre-class preparation, and safety specifications were strengthened. A diversified assessment system combining process evaluation and outcome evaluation was constructed with clear quantitative scoring standards. Teaching practice showed that the reform achieved phased results. A total of 23 college students’ innovation and entrepreneurship training programs were approved. The experiment teaching and assessment system was gradually improved, which effectively enhanced students’ experimental operation ability, innovative practice ability and course satisfaction. This paper provides a reference for the teaching reform of Cell Biology and related experiment courses in similar agricultural universities.