为探究蝴蝶兰耐低温的生理机制,本试验以蝴蝶兰品种大辣椒植株为试验材料,经过1、3、5和7 h的低温（5 ℃）处理后,分别测量其花蕾期、盛开期和衰败期叶片中的叶绿素、可溶性糖、可溶性蛋白、丙二醛（MDA）含量及电导率和A280值。结果表明,随着低温处理时间的延长,叶绿素总含量整体呈现先降低后升高的趋势;可溶性蛋白含量和可溶性糖含量变化不明显,其中可溶性蛋白含量在花蕾期呈下降趋势,衰败期的可溶性糖含量高于花蕾期和盛开期;MDA含量、电导率和A280随着低温处理时间的延长缓慢上升。叶绿素、MDA、电导率和A280的动态变化可反映蝴蝶兰受低温损失程度,而可溶性糖、可溶性蛋白与抗寒性相关性较低。
In order to explore the physiological mechanism of low temperature tolerance in Phalaenopsis aphrodite, this experiment used Da La Jiao plants of Phalaenopsis aphrodite varieties as experimental materials. After being treated with low temperature (5 ℃) for 1,3,5, and 7 hours, the chlorophyll,soluble sugars,soluble proteins, malondialdehyde (MDA) content, electrical conductivity, and A280 value in their leaves during the bud, blooming, and decline periods were measured. The results showed that with the extension of low-temperature treatment time, the total chlorophyll content showed an overall trend of first decreasing and then increasing. The changes in soluble protein content and soluble sugar content were not significant. The content of soluble protein showed a decreasing trend during the bud stage. The soluble sugar content during the decline period was higher than that during the bud and blooming periods. The MDA content, conductivity, and A280 slowly increased with the extension of low-temperature treatment time. The dynamic changes in chlorophyll, MDA, conductivity, and A280 ould reflect the degree of low-temperature loss in Phalaenopsis aphrodite, while the relationship between soluble sugars and soluble proteins and cold resistance is relatively small.
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