A questionnaire survey on graduates of Horticulture major was conducted in the context of rural revitalization construction. The survey results showed that graduates had a relatively high overall satisfaction with the professional practical teaching conditions and various practical teaching modules. There were also different proportions of respondents who think it was average, and some respondents were not satisfied. There was still a lot of room for improvement in the Horticulture major current practical teaching conditions and various modules. Most of the respondents who participated in the survey had a good understanding of agricultural non-point source pollution and the integrated management model of planting and breeding to achieve agricultural material circulation. 96.67% of the respondents believe that planting and breeding integration and ecological agriculture related content should be added to practical internship teaching. Based on the research results, specific suggestions had been put forward to optimize the reform of practical teaching of fruit poultry compound planting and breeding in Horticulture major, including increasing the integration of horticultural plant planting and breeding modules, breaking down barriers at the college level, strengthening the teaching staff, enhancing the construction of practical teaching bases, and strengthening innovation and entrepreneurship practical training. The purpose was to provide data support for the reform of practical teaching and training programs for applied horticultural talents, and to provide a reference for optimizing the practical teaching system for agricultural talents under the background of new agricultural science.