To scientifically and reasonably select the moisture content and packaging methods of tobacco stems, an investigation was conducted under natural aging conditions into the effects of three moisture contents (11%, 15%, and 18%), and two packaging methods (carton and burlap) on the moisture content, core temperature, appearance quality, and conventional chemical components of tobacco stems during the aging period (1 year). The results indicated that no mold growth was observed in the tobacco stems samples packed in burlap with moisture contents of 11% and 15%, while mold growth occurred in some tobacco stems samples packed in cartons at 15% moisture content and in both cartons and burlap at 18% moisture content. With the increase of moisture content in tobacco stems, the fluctuation of moisture content became larger, the color turned darker more significantly, and the risk of mold growth increased. The stability of moisture content in tobacco stems packed in cartons at 11% moisture content was superior to that packed in burlap. The core temperature was controlled within the range of 8.0~35.0 ℃, mainly influenced by the ambient temperature. The total sugar and reducing sugar in tobacco stems generally showed a decreasing trend, with a relatively larger decrease in high moisture content tobacco stems. The moisture content of reconditioned tobacco stems should be controlled around 11.0%, and it is advisable to use carton packaging.