Rice pot seedling machine transplanting effectively combines the characteristics of pot-shaped seedlings and carpet seedlings. It involves the precise transplantation of soil-bearing pot seedlings into the field using specialized machinery, offering advantages such as shortening the slow seedling recovery period and improving yield and stability. This article, based on the practice of rice pot seedling machine transplanting in Anqing, Anhui Province, introduces high-yield and high-efficiency cultivation techniques for rice pot seedling nursery and machine transplanting, covering aspects such as variety selection, seed treatment, seedling management, and pot seedling transplantation. In production, it is advisable to select varieties with suitable maturity and strong disease resistance, such as Haoliangyou 729 and Haoliangyou 985. Seed treatment measures, including sun drying and soaking, are implemented to improve seedling emergence rates. For seedling management, a flat and well-irrigated field is selected as the nursery. Using nursery trays measuring 60 cm in length and 30 cm in width, with 448 holes per tray. The required number of trays is 35 trays per 667 m2. After sowing, soil sealing treatment, scientific water management, and fertilization are carried out to cultivate standardized seedlings with a hole formation rate of ≥90%, uniform growth, and free from pests and diseases. During transplantation, the row spacing for pot seedling machine transplanting is set at 33 cm × 14 cm, with a planting density of 14 400 holes per 667 m2 and a transplanting depth of 1–2 cm. After machine transplanting, water management follows the principle of “shallow water initially, mid-term drying, and moist conditions later”, shallow water before seedling recovery, field drying when tillers reach 90% of the panicle number, intermittent irrigation during jointing and booting stages, alternating dry and wet conditions during the grain-filling stage, and water supply is cut off 7-10 days before harvest. 5–7 kg /667 m2 of urea during the tillering stage to promote tillering, 2–3 kg/667 m2 of urea + 3–5 kg/667 m2 of potassium chloride during the booting stage to protect panicles, and foliar fertilizers such as potassium dihydrogen phosphate during the grain-filling stage to increase grain weight. Pest, disease, and weed control prioritize prevention, with chemical control applied at specific stages, supplemented by physical and biological control. Pesticide application should avoid high temperatures and the flowering period. Harvesting is conducted timely on sunny days when 95% of the grains turn yellow and 33% of the rice stalks dry out. The grains are dried to a moisture content of 13.5%–14.5% before storage.